˴ASPԇELISA
ʹf
l ԇwⶨzyѪѪ{M(x)弰P(gun)Һwӱ˴ASPĺ
l Чڣ6(g)
l l2-8
l ԇЃHwоʹR\
(sh)(yn)ԭ
ԇвpwһAķø(lin)ԇ(yn)ELISAA(y)Ȱ˴ףASP@wİУμ(bio)(bio)(zhn)ƷHRP(bio)ӛęzyw(jng)^صϴ졣õTMB@ɫTMB^øĴD(zhun){(ln)ɫD(zhun)KSɫɫ\͘Ʒе˴ףASPP(gun)ø(bio)x450nm LyȣOD ֵӋ(j)Ʒ
ӱ̎Ҫ
1. ѪȫѪ(bio)ՈҜط2Сr(sh)4^ҹ1000gx20ȡ弴əzy?q)?bio)-20-80(yng)ⷴ(f)ڡ
2. Ѫ{EDTA鿹(bio)ɼ30犃(ni)2 - 8C 1000gx20犣?q)?bio)-20-80(yng)ⷴ(f)
3. M{A(y)PBS (0.01M, pH=7.4)_ϴMȥѪҺ{ѽļt(x)(hu)Ӱ표yY(ji)QغMĽMc(yng)wePBSһ㰴1:9we1gĽMƷ(yng)9mLPBSwweɸ(j)(sh)(yn)Ҫm(dng){(dio)ӛ]PBSм뵰øƄ벣{УڱϳĥM(jn)һѽM(x)Ԍ{ҺM(jn)г(f)ڡ{Һ5000gx5~10ȡzy
4. (x)B(yng)(bio)1000gx20ȡ弴əzy?q)?bio)-20-80(yng)ⷴ(f)
ע(bio)Ѫ(hu)ӰzyY(ji)Ѫ(bio)M(jn)д(xing)zy
(bio)̎
1. ˾ֻԇбؓ(f)؟(z)ʹԓԇɵĘӱؓ(f)؟(z)Ոʹʹǰֿ]ӱĿʹA(y)Ęӱ
2. (sh)(yn)ǰ(yng)A(y)y(bio)(bio)^(yng)(bio)M(jn)ϡʹϡጺĘ(bio)ԇеęzyӋ(j)r(sh)ٳ(yng)ϡጱ(sh)(bio)ʹ0.01mol/LPBSϡ(PH=7.0-7.2)
3. zӱfИӱ֮hM(jn)A(y)(sh)(yn)(yn)CЧעӱ
4. ʹûW(xu)ѽҺƂĽM{(x)ȡҺܕ(hu)ijЩW(xu)|(zh)댧(do)ELISA(sh)(yn)Y(ji)ƫ
5. ӱ鼚(x)B(yng)ԓӱɔ_^磺(x)B(ti)(x)(sh)ɘӕr(sh)gȣԿܴڙzyr
6. ijЩȻؽMԭ˼ؽM?yn)ca(chn)Ʒʹõęzyw@wƥzy
7. hʹrӱr(sh)g^Lܕ(hu)ڵ׃Ԍ(do)(sh)(yn)Y(ji)ƫ
Ҫδṩԇ
1. ø(bio)x450nm
2. ߾ȼӘ^0.5-10uL2-20uL20-200uL200-1000uL
3. 37
4. sˮȥxˮ
ԇнM
Q |
96 |
48 |
ע |
ø(bio) |
812l |
86l |
o |
(bio)(zhn)Ʒ |
0.3mL*6 |
0.3mL*6 |
o |
ӱϡҺ |
6mL |
3mL |
o |
zyw-HRP |
10mL |
5mL |
o |
20ϴ쾏_Һ |
25mL |
15mL |
fM(jn)ϡ |
A |
6mL |
3mL |
o |
B |
6mL |
3mL |
o |
KֹҺ |
6mL |
3mL |
o |
Ĥ |
2 |
2 |
o |
f |
1 |
1 |
o |
Է |
1(g) |
1(g) |
o |
ע
1. (bio)(zhn)ƷΞ飺1206030157.50 nmol/L
2. (jng)^(bio)z(yn)(bio)ֵԇṩęzy(ni)(sh)(yn)^ֱȡ50LӱϘӼ(dng)в֘ӱֵ^(bio)(zhn)Ʒȕr(sh)ØӱϡҺ(bio)M(jn)m(dng)ϡጺM(jn)Ќ(sh)(yn)
ע(xing)
1. (yn)Ҏ(gu)ĕr(sh)g͜ضM(jn)МԱC(zhn)_Y(ji)ԇʹǰ_(d)Ҝ20-25ʹúرԇ
2. ϴ岻_Ԍ(do)²(zhn)_ĽY(ji)ڼǰ_Mɿ׃(ni)Һw^вҪ
3. ךҺwָӡtӰODֵ
4. @ɫҺ(yng)ʟoɫܜ\ɫѽ(jng)׃{(ln)ĵҺʹ
5. ԇ͘(bio)ĽȾe(cu)`Y(ji)
6. ڃ͜r(sh)⏊(qing)ֱ䡣
7. ƽҜغٴ_ܷԷˮl
8. κη(yng)ԇܽ|Ư܄Ư܄ɢl(f)ď(qing)ҚwκƯ׳ɷֶ(hu)Ɖԇз(yng)ԇ
9. ʹ^ڮa(chn)Ʒ
10. ܂еĘƷ(yng)Ҏ(gu)ij̎Ʒ͙zyb
ԇ(zhn)
ԇЏحh(hun)ȡ(yng)Ҝƽʹ
20ϴ쾏_Һϡጣsˮ120ϡ120ϴ쾏_Һ19sˮ
E
1. Ҝƽ20minXȡlʣlԷܷŻ4
2. O(sh)Ø(bio)(zhn)Ʒ͘ӱ(bio)(zhn)ƷӲͬȵĘ(bio)(zhn)Ʒ50L
3. ӱyӱ50Lհײ
4. հ⣬(bio)(zhn)Ʒ͘ӱÿ^øHRP(bio)ӛęzyw100L÷Ĥס(yng)37ˮԡ偻60min
5. ȥҺwˮĸÿӝMϴҺ350Lo1min˦ȥϴҺˮĸ؏(f)ϴ5ΣҲϴC(j)ϴ壩
6. ÿAB50L37ܹ15min
7. ÿKֹҺ50L15min(ni)450nmL̎yODֵ
(sh)(yn)Y(ji)Ӌ(j)
y(bio)(zhn)ƷODֵM(bio)(bio)(zhn)Ʒĝֵv(bio)(bio)P(gun)ܛL(bio)(zhn)õֱؚwƷODֵ뷽̣Ӌ(j)Ʒ
ԇ
1. zy3.75 nmol/L C 120 nmol/L
2. `ȣ͙zyС0.1 nmol/L
3. خԣcԽY(ji)(gu)サ淴(yng)
4. ؏(f)ԣ(ni)׃ϵ(sh)С10% g׃ϵ(sh)С15%
f
1. ڬF(xin)ЗlƌW(xu)g(sh)ˮƽв܌й؛ṩԭM(jn)ȫbca(chn)Ʒܴһ|(zh)g(sh)L(fng)U(xin)
2. KČ(sh)(yn)Y(ji)cԇЧ(sh)(yn)ߵP(gun)Լ(dng)r(sh)Č(sh)(yn)h(hun)P(gun)Ո(w)(zhn)Ę(bio)
3. ͬεͬһa(chn)Ʒܕ(hu)Se磺zy`Լ@ɫr(sh)gȣՈ(j)ԇЃ(ni)fM(jn)Ќ(sh)(yn)W(wng)վӰfH
4. ֻȫʹñԇԇܱCzyЧ̵ܻĮa(chn)ƷֻЇ(yn)رԇеČ(sh)(yn)fŕ(hu)õѵęzyY(ji)
}
(sh)(yn)ЧãՈr(sh)@ɫY(ji)ðlδʹԇƱ棬Ȼ(lin)ϵҹ˾g(sh)֧֞Q}ͬr(sh)ҲԅYϣ
} |
ԭ |
Q |
(bio)(zhn) |
ȡϴ첻 |
ֵȡϴ |
Һ_ |
zУҺ |
|
ܶȵ |
ϴ첻 |
fҪϴͽ |
ֺȡԇ |
ֻȡԇ |
|
؏(f)^Ĥ |
ʹüӘҪQµ^ʹµĤ |
|
ӘӲ_ |
zУҺ |
|
O.Dֵ |
ÿӵԇ_ |
УҺ_ԇ |
r(sh)g_ |
CĜr(sh)g |
|
ضȲ_ |
ԇҪƽҜزC(zhn)_Ĝض |
|
ø(bio)ӛʧЧ |
ͨ^ø(bio)ӛ͵ɫ(yng)Ѹ@F(xin)z |
|
]мKֹҺ |
f(sh)(yn)EKֹҺ |
|
x(sh)r(sh)gx(sh) |
f]x(sh)r(sh)g(ni)x(sh) |
|
ӱֵ |
_Ęӱ淽ʽ |
_ӱʹrӱM(jn)Ќ(sh)(yn) |
_Ęӱռ̎ |
ȡ_Ęӱռ̎ |
|
y|(zh)ژӱк |
ʹrӱ؏(f)(sh)(yn) |
˽M(jn)ژ(bio)(zhn)Ʒhttp://www.shhyswsj.com/hyshsj2011-SonList-578253/
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